Used applied biosystems 3xl genetic analyzer 2020339. Sequencing software applied biosystems sequence scanner. Applied biosystems 33xl genetic analyzers getting started guide ix preface how to use this guide purpose of this guide the applied biosystems 33xl gene tic analyzers getting started guide provides stepbystep instructions for pr eparing and analyzing a sample. Upgrade of applied biosystems genetic analyzers, 3 series. Applied biosystems 33xl genetic analyzers maintenance, troubleshooting, and reference guide pn 4352716 contains information on maintaining the instrument and computer system, troubleshooting tables to diagnose performance failures, and advanced reference content. Applied biosystems stretch 373a abi dna sequencer ebay. Gel electrophoresis is the movement of charged molecules through a gel solution such as polyacrylamide in an electrical field. Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Get compliance and validation services for applied biosystems and ion torrent instruments including nextgeneration and sanger sequencing platforms as well as their connected computers and electronic. It has 12 configurable microfluidic flowchips with 6 independent lanes each to enable flexibility. Generaluberholter sequencer abi 3 abi 3xl vom profi zu verkaufen.
Most importantly, as research throughput increases, the instrument can be upgraded to 16capillaries. You can also download our guide to dna sequence analysis. System is complete with computer and original disks. Applied biosystems 33xl genetic analyzers using data collection software v3. The abi 3 is another ideal dna sequencer for low to midthroughput labs. Below are some of the key features and benefits of the abi 3xl. To learn more about current applications using sanger sequencing and to download the. This applied biosystems stretch 373a dna sequencer is in real good condition and guaranteed to functions 100%. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The 3xl 16capillary array system is used to electrophoretically analyze samples following. The applied biosystems 3 and 3xl genetic analyzers includes. To speak with a representative, please fill out the contact form below or call us directly. You may have noticed our june 2017 statement, where we informed you that.
The applied biosystems 3xl is a 16capillary electrophoresis system for genetic analysis. Solid 5500xl sequencing by oligonucleotide ligation and detection developed by life technologies and made available in 2006, the solid 5500xl sequencer is the only next generation platform to sequence via primer ligation. Designed for mediumthroughput applications, it is capable of sequencing and fragment analysis including microsatellite, aflp, loh, and snp validation and screening. Pcr products were analysed on an abi prism 3 xl genetic analyzer applied biosystems with a 50 cm capillary and pop7 applied biosystems. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Large and small sequencing projects are easily accommodated for internal and external customers. Save target as to download a pdf version of the document to a destination. For more information regarding licenses, please contact the director of licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, usa. Across injections, the data indicates that high standard deviation values were obtained from the following loci. Get compliance and validation services for applied biosystems and ion torrent instruments including nextgeneration and sanger sequencing platforms as.
Abi 3xl dna sequencer the abi 3xl dna sequencer is a 16capillary instrument that offers highquality data, efficient sample processing, and 24hour unattended operation. It can process samples from 96 or 384 well plates, with mixed applications run on a single array. Applied biosystems 3 and 3xl dna analyzers key features 16 capillaries for the 3 xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup. Be sure to have these on hand when you are performing experiments with the 33xl genetic analyzers.
Applied biosystems 3 genetic analyzer thermo fisher. You can open and download a pdf using adobe acrobat. Applied biosystems 37303730 xl our scientific research. Applied biosystems 3 3xl genetic analyzers support. Applied biosystems 3 3xl genetic analyzers maintenance, troubleshooting, and reference guide v preface how to use this guide purpose of this guide the applied biosystems 33 xl genetic analyzers maintenance, troubleshooting, and reference guide provides instructions for maintaining the applied biosystems 3 3xl genetic analyzers. Explore the getting started and troubleshooting sections for solutions to top inquiries and common problems.
Applied biosystems cycle sequencing kits available for dye terminator. Price list for selected equipment, supplies and reagents. These machines use capillary electrophoresis, which is a significant advantage over slabgel based technologies. Applied biosystems 3xl is just one of the fantastic products created by a company who has a long standing tradition of providing excellence in life science instruments, reagents and software find online auctions and classified ads for applied biosystems products on the labx marketplace. To describe the standardized laboratory protocol for molecular subtyping of salmonella enterica serotype enteritidis. Abi 3xl sequencer available for sale at the lab world group. Seqtrace is designed for sequencing projects that require converting trace files directly into highquality. This increases lab productivity 4fold, without needing to buy another sequencer. Before each run, the capillaries are automatically replenished with fresh electroosmotic flow eof suppression polymer. Level 1 dna sequencing on applied biosystems 3xl genetic analyzer. Applied biosystems abi 3xl 16capillary array genetic. To describe the standardized laboratory protocol for molecular subtyping of salmonella enterica serotype typhimurium. Browse ads and contact the seller directly or request a quote for more details. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years.
Applied biosystems 3 3xl genetic analyzers getting started guide ix preface how to use this guide purpose of this guide the applied biosystems 3 3xl gene tic analyzers getting started guide provides stepbystep instructions for pr eparing and analyzing a sample. The applied biosystems 33xl genetic analyzer system includes both the instrument and the computeryou cannot start or restart one without starting the other. Applied biosystems 7500 getting started manual pdf. Applied biosystems 33xl genetic analyzers supporttroubleshooting applied biosystems 37303730xl dna analyzers support applied biosystems 35003500xl genetic analyzers support applied biosystems seqstudio genetic analyzer support. Core facility operates two applied biosystems 3xl sequencers. Applied biosystems is one of the various brands under the life technologies brand of thermo fisher scientific corporation. The applied biosystems 3730 and 3730xl dna analyzer includes patented technology licensed from hitachi, ltd. Applied biosystems 33xl genetic analyzers getting started. Dna sequence analysis bioinformatics genomics miami university.
Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Our facility is fully equipped for sanger dna sequencing, next generation. Dna sequencing by capillary electrophoresis by applied biosystems. Manufacturer of applied biosystems 3 genetic analyzer. Wait until the desktop appears and all programs have finished loading hard drive light on computer stops flashing. Increased sensitivity for amplified str alleles on.
The applied biosystems 3730xl dna analyzer can be used for dna fragment analysis applications such as microsatellites, aflp, snp analysis, mutation detection and traditional dna sequencing. Applied biosystems 3 and 3xl dna analyzers key features 16 capillaries for the 3xl and. The sequencing is done on 3730xl with bigdye taq fs terminator v 3. The genotyping and fragment sizing are done on 3xl please consult our recommendations for template preparation to avoid failures. Capillary electrophoresis guides and catalog download. It came from a local lab that was upgrading equipment and we can accept pay pal or your credit card. The brand is focused on integrated systems for genetic analysis, which include computerized machines and the consumables used within them such as reagents in 2008, a merger between applied biosystems and invitrogen was finalized. The purchase price of the applied biosystems 33xl genetic analyzer includes a grant of a limited, nontransferable license under u. Setupandanalysisusingyourowncustommlstscheme 1 introduction this tutorial illustrates the steps needed to set up your own mlst schema, e. Abi prism dna sequencing analysis or abi prism genescan analysis software. Applied biosystems 3730xl dna analyzer 1979b dna duration. View, edit, print and export data generated using ab37030xl and other applied biosystems genetic. However, this wizard was dropped from the 3730xl and the 3xl sequencers. David fegredo medical scientistpathwestaustralia the abi 3 and 3xl genetic analyzers are the newest in a range of analyzers produced by abi.
The applied biosystems 3100 is an automated, highthroughput, capillary electrophoresis system used for analyzing fluorescently labeled dna fragments find online auctions and classified ads for applied biosystems products on the labx marketplace. Used applied biosystems 3xl genetic analyzer 2030217. Applied biosystems 33xl genetic analyzers maintenance, troubleshooting, and reference guide v preface how to use this guide purpose of this guide the applied biosystems 33 xl genetic analyzers maintenance, troubleshooting, and reference guide provides instructions for maintaining the applied biosystems 33xl genetic analyzers. Similarly as a single capillary sequencer, abi prism 310 mentioned above, also 3 series of genetic analyzers abi3 and abi3xl, represent instruments that have outlived their expectations. Sequencing results are placed on a server for download from this web site to your. Check out the most popular applied biosystems products for genetic analysis. Polymers can be used on the applied biosystems 3 and 3xl genetic analyzers as the separation matrix. Applied biosystems 3 genetic analyzer applied biosystems 3xl genetic analyzer. Applied biosystems 3730xl dna analyzer 1979b dna youtube.
Scope to provide pulsenet participants with a single protocol for performing mlva of s. Refurbished applied biosystems abi 3xl 16capillary array genetic analyzerdna sequencer analyzerdna sequencer pn 6280030 complete with new laser, windows xp pc, data collection 3. Within injections, the data shows no markers andor alleles with high standard deviation values exceeding 0. Turn on the instrument by pressing the power button. Applied biosystems 3xl genetic analyzer get latest price a offering 16capillary throughput, advanced automation capabilities and superior performance, this system is ideal for mediumthroughput laboratories that need streamlined setup and 24hour unattended operation. Bid history for genetic analyzer, hitachi applied biosystems mdl 3xl hc1252. Once again on the applied biosystems 3xl sequencer service. The fully automated system can run hundreds of samples per day. The applied biosystems 5500xl solid sequencer provides embedded sequencing controls to maximize data quality and performance. Browse through the guides and tools section to access comprehensive productrelated support resources.
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